Biological Information
Background Information:
The GRM5 Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. L-glutamate) based activation of GRM5 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GRM5 , a naturally Gq coupled GPCR. Upon activation of GRM5 , the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Glutamate (Metabotropic)
Sub Family:
Class C
Coupling:
Gq
Protein Name:
mGlu5
Protein Aliases:
protein phosphatase 1, regulatory subunit 86
Accession Number:
NM_000842.3
UniProt Number:
P41594
Gene Name:
GRM5
Gene ID:
2915
Gene Aliases:
MGLUR5|GPRC1E|mGlu5|PPP1R86
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
Calcium Flux
Detection Method:
Chemiluminescence
Additional Information
Brand:
Eurofins DiscoverX®