Biological Information

Background Information:

The GRM5 Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. L-glutamate) based activation of GRM5 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GRM5 , a naturally Gq coupled GPCR. Upon activation of GRM5 , the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Glutamate (Metabotropic)

Sub Family:

Class C

Coupling:

Gq

Protein Name:

mGlu5

Protein Aliases:

protein phosphatase 1, regulatory subunit 86

Accession Number:

NM_000842.3

UniProt Number:

P41594

Gene Name:

GRM5

Gene ID:

2915

Gene Aliases:

MGLUR5|GPRC1E|mGlu5|PPP1R86

Target Species:

Human

Cell Background:

U2OS

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

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Assay Information

Assay Type:

Cell Based

Assay Measures:

Calcium Flux

Detection Method:

Chemiluminescence

Additional Information

Brand:

Eurofins DiscoverX®